Streptococcal Pyrogenic Exotoxin B-Induced Apoptosis in A549 Cells Is Mediated through v 3 Integrin and Fas
نویسندگان
چکیده
Streptococcal Pyrogenic Exotoxin B-Induced Apoptosis in A549 Cells Is Mediated through v 3 Integrin and Fas Wan-Hua Tsai, Chia-Wen Chang, Yee-Shin Lin, Woei-Jer Chuang, Jiunn-Jong Wu, Ching-Chuan Liu, Pei-Jane Tsai, and Ming T. Lin* Institute of Basic Medical Sciences and Departments of Biochemistry, Microbiology and Immunology, Medical Laboratory Science and Biotechnology, and Pediatrics, National Cheng Kung University Medical College, Tainan, Taiwan; National Applied Research Laboratories, National Laboratory Animal Center, Taipei, Taiwan; and Institute of Medical Sciences, School of Medicine, Tzu Chi University, Hualien, Taiwan
منابع مشابه
Procaspase 8 and Bax are up-regulated by distinct pathways in Streptococcal pyrogenic exotoxin B-induced apoptosis.
We have previously identified integrin alpha(v)beta(3) and Fas as receptors for the streptococcal pyrogenic exotoxin B (SPE B), and G308S, a mutant of SPE B that binds to Fas only. In the current study we found that after binding to alpha(v)beta(3), SPE B stimulated the tyrosine phosphorylation of JAK2 and STAT1. STAT1 tyrosine phosphorylation was inhibited by a JAK2 inhibitor, AG490, short int...
متن کاملStreptococcal pyrogenic exotoxin B-induced apoptosis in a549 cells is mediated by a receptor- and mitochondrion-dependent pathway.
It has been shown that streptococcal pyrogenic exotoxin B (SPE B) can induce cells to undergo apoptosis. The present study is to dissect the role of SPE B protease and SPE B protein in the apoptotic process of A549 cells and to elucidate the SPE B-induced apoptotic pathway. Recombinant SPE B (rSPE B) and C192S, a mutant of SPE B without protease activity, were expressed in Escherichia coli and ...
متن کاملThe superantigenic activity of streptococcal pyrogenic exotoxin B is independent of the protease activity.
The nature of the mitogenic activity of pyrogenic streptococcal exotoxin B, also known as streptococcal cysteine protease, has been debated in the literature. Streptococcal exotoxin B has been shown to cleave interleukin-1beta precursor and create biologically active interleukin-1beta, a major cytokine mediating inflammation and shock. This activity could mimic the mitogenicity and cytokine rel...
متن کاملMatrine inhibits diethylnitrosamine-induced HCC proliferation in rats through inducing apoptosis via p53, Bax-dependent caspase-3 activation pathway and down-regulating MLCK overexpression
The proliferation of hepatocellular carcinoma (HCC) cells is one of the leading causes of liver cancer mortality in humans. The inhibiting effects of matrine on HCC cell proliferation have been studied, but the mechanism of that inhibition has not been fully elucidated. Since, apoptosis plays an important role in HCC cell proliferation. We examined the apoptosis-inducing effect of matrine on tu...
متن کاملStreptococcal pyrogenic exotoxin B induces apoptosis and reduces phagocytic activity in U937 cells.
Treatment of U937 human monocyte-like cells with Streptococcus pyogenes led to an induction of apoptosis in these cells. A comparison between the wild-type strain and its isogenic protease-negative mutant indicated that the production of streptococcal pyrogenic exotoxin B (SPE B), a cysteine protease, caused a greater extent of apoptosis in U937 cells. Further study using purified SPE B showed ...
متن کامل